dna cdna template (Thermo Fisher)

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Thermo Fisher dna cdna template
Dna Cdna Template, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/template+dna+cdna/DNA/pmc12681265-241-1-29
Average 99 stars, based on 1 article reviews

dna cdna template - by Bioz Stars, 2026-09

99/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Exosomal miR‑194 from adipose‑derived stem cells impedes hypertrophic scar formation through targeting TGF‑β1.
Article Snippet: Hypertrophic scars, which result from aberrant fibrosis and disorganized collagen synthesis by skin fibroblasts, emerge due to disrupted wound healing processes.. These scars present significant psychosocial and functional challenges to affected individuals.. The current treatment limitations largely arise from an incomplete understanding of the underlying mechanisms of hypertrophic scar development.

Article Title: Exosomal miR‑194 from adipose‑derived stem cells impedes hypertrophic scar formation through targeting TGF‑β1
Article Snippet: Transcription Kit, cat. no. 205311; Qiagen GmbH). .. The reaction mix of qPCR included 50 ng template DNA/cDNA, 0.5 μl of each primer, 2 μl PCR buffer, 1 U of Taq DNA polymerase and 0.3 μl PowerTrack SYBR Green Master Mix (cat. no. A46109; Thermo Fisher Scientific, Inc.), adjusted to 20 μl with deionized water. .. Thermal cycling conditions comprised an initial denaturation at 95°C for 5 min, followed by 35–40 cycles of denaturation at 95°C for 30 sec, primer-specific annealing and extension at 72°C for 60 sec, concluding with a final extension at 72°C for 5 min. qPCR was performed with Sybr Green on an Exicycler 96 instrument (Bioneer Corporation).

Polymerase Chain Reaction:

Article Title: Development and diagnostic performance of a sensitive multiplex pneumonia pathogen identification assay for emergency department patients with pneumonia.
Article Snippet: .. Each 25 μL multiplex PCR reaction contained 4 μL of template DNA/cDNA, 80 nM of each pathogenspecific primer, 1 μM of UF/UR primers, 16 nM RNase P (RP) primer pairs, 2.5 mM MgCl2, 200 μM of each deoxyribonucleotide triphosphate, 1× AmpliTaq Gold Buffer, and 0.625 units of AmpliTaq Gold DNA Polymer ase (Applied Biosystems, Foster City, CA, USA). ..

Article Title: Genome-wide comprehensive characterization and expression analysis of TLP gene family revealed its responses to hormonal and abiotic stresses in watermelon (Citrullus lanatus).
Article Snippet: Thaumatin-like protein (TLP) is the well-known sweetest protein which plays a crucial role in diverse developmental processes and different stress conditions in plants, fungi and animals.. The TLP gene family is extensively studied in different plant species including crop plants.. Watermelon (Citrullus lanatus) is an important cucurbit crop cultivated worldwide; however, the comprehensive information about the TLP gene family is not available in watermelon.

Article Title: Development and diagnostic performance of a sensitive multiplex pneumonia pathogen identification assay for emergency department patients with pneumonia
Article Snippet: .. Each 25 μL multiplex PCR reaction contained 4 μL of template DNA/cDNA, 80 nM of each pathogen-specific primer, 1 μM of UF/UR primers, 16 nM RNase P (RP) primer pairs, 2.5 mM MgCl 2 , 200 μM of each deoxyribonucleotide triphosphate, 1× AmpliTaq Gold Buffer, and 0.625 units of AmpliTaq Gold DNA Polymerase (Applied Biosystems, Foster City, CA, USA). ..

Article Title: Bacterial 16S rRNA/rDNA Profiling in the Liquid Phase of Human Saliva
Article Snippet: .. The PCR reaction mixture (50 μl) contained 0.1 to 1 ng of template DNA/cDNA, 200 μM of each dNTP, 40 pM of each primer, 4.0 mM of MgCl 2 , 5 μL of 10 × PCR buffer II, and 2.5 U of Taq DNA polymerase (PE Applied Biosystems). ..

SYBR Green Assay:

Multiplex Assay:

Article Title: Development and diagnostic performance of a sensitive multiplex pneumonia pathogen identification assay for emergency department patients with pneumonia
Article Snippet: .. Each 25 μL multiplex PCR reaction contained 4 μL of template DNA/cDNA, 80 nM of each pathogen-specific primer, 1 μM of UF/UR primers, 16 nM RNase P (RP) primer pairs, 2.5 mM MgCl 2 , 200 μM of each deoxyribonucleotide triphosphate, 1× AmpliTaq Gold Buffer, and 0.625 units of AmpliTaq Gold DNA Polymerase (Applied Biosystems, Foster City, CA, USA). ..

Polymer:

Amplification:

Article Title: HIV-1 Nef Induces Hck/Lyn-Dependent Expansion of Myeloid-Derived Suppressor Cells Associated with Elevated Interleukin-17/G-CSF Levels
Article Snippet: .. The reaction mixture contained 5 μl SYBR select master mix (Life Technologies), 0.5 μl of each primer, at 10 pmol/liter and 2 μl template DNA (cDNA) in 2.0 μl RNase-free H 2 O. Amplification was for 40 cycles (15 s at 95°C and 1 min at 60°C) with an AB Applied Biosystems ViiA7 instrument (Life Technologies). ..

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